T2a-creert2
Web品系名： C57BL/6JSmoc- Them1 (2A-tdTomato)Smoc. 品系状态 ：胚胎冻存 目录号：NM-KI-210124. 应用领域： 该小鼠在Th基因座表达报告基因 ( tdTomato)。. 该报告基因在儿茶酚胺能细胞中表达，可用于研究多巴胺能细胞功能、帕金森病和其他神经退行性疾病。. 小鼠. Web021189 B6.Cg- Pvalb tm1.1(cre/ERT2)Hze /J Pvalb-T2A-CreERT2-D (Pvalb-2A-CreERT2-D) mice have Cre-ERT2 fusion gene expression directed to Pvalb -expressing cells by the …
T2a-creert2
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Webp-EF1a-CreERT2-3Xflag-T2A-eBFP2 (Plasmid #170186) Print Enlarge View all sequences Purpose This Cre-ERT2 expressing construct can be used to inducibly recombine loxp … WebJul 16, 2024 · A 3’ knock-in pipeline and the characterization of TgKI(krt92-p2A-EGFP-t2A-CreERT2). With the aim to generate knock-in zebrafish lines for both cellular labelling and lineage tracing, we designed our vector templates encompassing a fluorescence protein and different Cre recombinase linked by two self-cleavable 2A peptide sequences (p2A and …
WebpCAGGS-mTagBFP2-T2A-iCreERT2 (PV) Plasmid #128171 Purpose Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus) Depositor Mario Capecchi Insert mTagBFP2-T2A-iCreERT2 Use Cre/Lox Tags Expression Mammalian Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pCAGGS-mTagBFP2-T2A-iCreERT2 … WebAug 11, 2024 · Next, variants of T2A-iCre-ER T2 were constructed, with variations at the N-terminus of iCre-ER T2 after the ribosome skip. The sfGFP-iCre-ER T2 was included as a control. The mTagBFP2 was initially cloned in as a transfection marker to normalize for transfection efficiency.
WebTamoxifen诱导Cre-ERT2小鼠 使用指南 Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物） … WebJun 17, 2015 · To enlarge the pool of existing CreERT2-driver lines, we performed a genome-wide gene trap screen using a Tol2-based mCherry-T2a-CreERT2 (mCT2aC) gene trap vector. This cassette consists of a splice acceptor and a mCherry-tagged variant of CreERT2 which enables simultaneous labeling of the trapping event, as well as CreERT2 expression …
WebMar 1, 2024 · Using a TbetZsGreen-T2A-CreERT2 driver, a subset of T follicular helper cells (Tfh) was revealed to express the proinflammatory cytokine IFN-γ in germinal centers …
did fenrir have childrenWeb030197 B6;129S- Pdyn tm1.1(cre/ERT2)Hze /J Pdyn-T2A-CreERT2-D knock-in mice express tamoxifen-inducible Cre recombinase from the endogenous prodynorphin promoter/enhancer elements. When induced, CreERT2 expression/activity is observed mainly in central amygdalar nucleus, paraventricular hypothalamic nucleus, nucleus … did fergie attend queen elizabeth\u0027s funeralWebJun 1, 2024 · The Cre recombinase was fused to a modified estrogen receptor (CreERT2) and inserted to the end of the open reading frame of the Tph1 gene at exon 11 with a self-cleaving peptide, T2A (Figure 1Aa and b). The recombinant transcript with T2A (Tph1-T2A-CreERT2) allows the peptide to be cleaved into 2 proteins, TPH1 and CreERT2. did fenty beauty have any failuresWebThe Cre recombinase was fused to a modified estrogen receptor (CreERT2) and inserted to the end of the open reading frame of the Tph1 gene at exon 11 with a self-cleaving peptide, T2A (Figure 1Aa and b). The recombinant transcript with T2A (Tph1-T2A-CreERT2) allows the peptide to be cleaved into 2 proteins, TPH1 and CreERT2. did ferdinand magellan have a wifeWebThe Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the … did ferdinand magellan find the spice islandsWebPlasmid pAAV-Sox2-CreERT2 from Dr. Lin He's lab contains the insert P2A-CreERT2 flanked by Sox2 homology arms and is published in Cell Rep. 2024 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2024.05.103. This plasmid is available through Addgene. did fergie go to the funeralWebMar 3, 2024 · zsGreen–T2A–CreERT2–SV40 poly(A)–LoxP–Neo–LoxP, was constructed by Gibson cloning (Gibson et al., 2009). This cassette was then used to replace the Atoh7 ORF in a genomic DNA fragment captured by BAC recombineering (Fu et al., 2009). The final construct contains a 5.3 kb 5’ arm, the zsGreen–T2A–CreERT2–SV40 poly(A)–LoxP– did fergie cheat on andrew