Pef6/v5-his b
WebInvitrogen™ pEF6/V5-His A, B, & C Mammalian Expression Vectors. Contains strong promoter for high-level expression in mammalian cells. Manufacturer: Invitrogen™ … WebAll pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the …
Pef6/v5-his b
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WebApr 30, 2024 · PCR products were inserted into pEF6/V5-His B vector (Invitrogen) at EcoRV sites using In-Fusion HD Cloning Kit (TaKaRa) or NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA). The plasmids of dNlgns were modified by replacing V5-His tag with FLAG tag. Therefore, we obtained the following five plasmids: pEF6-dNlgn1-FLAG, … WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown
WebpEF6 V5-His TOPO® TA Vector Kit pEF6/V5-His TOPO® TA Vector Kit, included in the MembranePro™ Functional Protein Expression Kit and also available separately (see page 29), contains the reagents listed below. Upon receipt, store the vector kit at –20°C. Reagent Composition Amount pEF6/V5-His-TOPO® vector 10 ng/µL plasmid DNA 20 µL http://www.biofeng.com/plus/view.php?aid=13720
WebThermo Fisher mammalian expression pef6 v5 his topo plasmid vector Mammalian Expression Pef6 V5 His Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
WebDensitometric analysis of (A) LRP6 phosphorylation and (B) total LRP6 content in cells treated with the indicated concentrations of Rspo2-2F, Rspo2-2F/Q70R and/or Wnt-3a for 1, 6 or 20 h prior to processing for immunoblotting. Cell lysates also were immunoblotted for HSP70 as a loading control. hockey hall of fame by yearWebiv Kit Contents and Storage Shipping and Storage pVAX1™ and pVAX1™/lacZ are shipped at room temperature. Upon receipt, store vectors at –20°C. Kit Contents 20 µg each of pVAX1™ and pVAX1™/lacZ, are supplied at 0.5 μg/μL in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a total volume of 40 μL. htck poldaWebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。 htc knockouts pieWebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one … hockey hall of fame book of goaliesWebContents 20 μg each pEF6/Myc-His A, B, and C, lyophilized 20 μg pEF6/Myc-His/lacZ, lyophilized Shipping/Storage Lyophilized plasmids are shipped at room temperature and … htck3623sWebγ-Aminobutyric acid type A receptors (GABAARs) mediate primarily inhibitory synaptic transmission in the central nervous system. Following fast-paced activation, which provides the selective flow of mainly chloride (Cl−) and less bicarbonate (HCO3−) ions via the pore, these receptors undergo desensitization that is … htc key seat cutterWebJun 2, 2014 · WT and mutant SOX11 cDNAs were PCR amplified and cloned into the pEF6/V5-His B mammalian expression vector (Life Technologies) using the In-Fusion PCR Cloning Kit (Clontech Laboratories), and also ... htc keys limited co. ltd