Oligomycin-antimycin
WebAs an example, consider a classical experiment assessing mitochondrial function by studying effects of oligomycin, FCCP, and rotenone/ antimycin A on OCR (Fig. 13.3). Oligomycin inhibits the ATP synthase so any oxygen consumption remaining in the presence of this compound is due to uncoupled and nonmitochondrial respiration. FCCP … Web26. nov 2024. · This study sought to elucidate how oligomycin, an ATP synthase blocker, leads to underestimation of maximal oxygen consumption rate (maxOCR) and spare …
Oligomycin-antimycin
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WebJurkat cells were suspended in calcium-free BSS at pH 7.2 or 7.8, and after treatment with 0.12 M oligomycin (A, B, D, and E), were exposed to 2 M CCCP (A and D) or 0.5 g/ml antimycin A (B and E). Web16. nov 2014. · Oligomycin A (1uM), FCCP (200nM for PC3-Epi and PC3-EMT, and 400nM for PrECs), rotenone (2uM), antimycin A (2uM), and 2-DG (100mM) were used to evaluate mitochondrial respiratory capacity and glycolytic capacity. The data were normalized by the amount of protein present in each well.
WebOligomycin. In cells given oligomycin and FCCP, changes in ECAR before and after injection of electron transport chain inhibitors may indicate whether CO2 is a contributing … WebTransfer to the two control situations (oligomycin or antimycin + CCCP inhibition) of the positive and negative peaks, defined in the basal state, allowed for calculating the proportion of fluorescent sperm (# sperm in positive peak/total # sperm analyzed) and MMP (according to Nernst law, taking as zero signals the fluorescence value under ...
Web13. nov 2024. · Similar to known OXPHOS inhibitors (Rotenone for complex I, Antimycin A for complex III, and Oligomycin A for complex V in OXPHOS pathway), Baicalein dose dependently induces ATF4 expression ... WebOligomycin (Synonyms: 寡霉素) Oligomycin. (Synonyms: 寡霉素) Oligomycin 是一种抗真菌抗生素，是 H+-ATP 合成酶的抑制剂。. Oligomycin 断氧化磷酸化和电子传递链。. …
Web26. dec 2024. · Confocal imaging of mitophagy with mito-mKeima. WT or PINK1 KO HeLa cells stably expressing YFP-Parkin (not shown) and mito-mKeima were treated with dimethyl sulfoxide (DMSO) or oligomycin+Antimycin A+Q-VD (OAQ) for 7 hr or 24 hr. mt-mKeima excited at 488-nm was artificially colored as green and mito-mKeima excited at 561-nm … cruise a home houseboatsThey have use as antibiotics. Oligomycin A is an inhibitor of ATP synthase. In oxidative phosphorylation research, it is used to prevent state 3 (phosphorylating) respiration. Oligomycin A inhibits ATP synthase by blocking its proton channel (FO subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flo… cruise alaska small shipWeb19. jan 2024. · Current approaches to initiate mitophagy include acute dissipation of the mitochondrial membrane potential (ΔΨ m) by mitochondrial uncouplers (for example, … cruise air conditioner usedWebOligomycin and antimycin A prevented NO-induced apoptosis in a dose-dependent fashion by preventing cytochrome C release independent of Bcl-2 expression. However, neither compound affected the up-regulation of Bax protein. On the one hand, oligomycin treatment was not accompanied by a decline in DeltaPsi. build simulator torrentWebThe complex I inhibitor rotenone (Chu et al., 2013), the complex III inhibitor antimycin A (McLelland et al., 2014), the potassium ionophore valinomycin, and the ATP synthase … cruise alaska best time weatherWeb12. apr 2024. · Rotenone, antimycin A were purchased from Vinci-Biochem (Vinci, Italy). NADH, ... The inhibition of ATP synthase by oligomycin ruled out any possible concomitant effect on respiratory activity, while the absence of any effect after the addition of the protonophore FCCP confirmed that mitochondria were uncoupled. Moreover, the … cruise alaska march 2023WebJurkat cells were suspended in calcium-free BSS at pH 7.2 or 7.8, and after treatment with 0.12 M oligomycin (A, B, D, and E), were exposed to 2 M CCCP (A and D) or 0.5 g/ml … cruise all inclusive package