WebDec 2, 2005 · The inhibition of Rad51 foci observed in p53wt HCT116 cells correlates with our results reporting p53-dependent Rad51 downregulation in this cell line (Fig 2C). The specific contribution of p53-dependent mechanisms in repressing HR remains to be established; however, cooperative efficient repression of HR by p53 may be required. WebApr 21, 2024 · b HCT116 p53 KO cells were transfected with Flag-p53 plasmids, and then cells were treated as in Fig. 1a. Whole-cell extracts were analyzed by western blot with …
Flavopereirine Suppresses the Growth of Colorectal Cancer Cells …
WebTo further explore the inhibitory effects of PNAP-6 on colon cancer cells, the viability of HCT116 (p53 wild type) and HT-29 (p53 mutant) cells were examined by MTT assay … WebJan 12, 2024 · Wild-type HCT116 cells (HCT116/p53 +/+) and p53 knockout HCT116 cells (HCT116/p53 −/−) were a gift from Dr. Yao Lin (Fujian Normal University, Fujian, China) … ray winstone sweeney todd watch
Regulation of tumor suppressor p53 and HCT116 cell …
WebHCT116 p53–/– cells as well as a derivative cell line expressing the p53-Luc fusion were tested and the data presented are an average of three independent experiments. Activation of the p53 protein protects the organism against the propagation of cells that carry damaged DNA with potentially transforming mutations. MDM2, a p53-specific E3 ... WebHCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for … WebAug 12, 2016 · Loss of p53 function in cancer cells contributes to tumor progression in the presence of fibroblasts. (A and B) Relative expression of p53 and its downstream targets were determined in HCT116 +/+ and HCT116 −/− cells using qRT–PCR (A) (n = 3/group) and western blotting (B); *P < 0.05 versus HCT116 +/+ cells.(C) HCT116 +/+ or HCT116 … simply to impress yard signs