WebJun 15, 2024 · Purpose: Low molecular weight cyclin E (LMW-E) detected by Western blot analysis predicts for reduced breast cancer survival; however, it is impractical for … WebCyclin E, a key mediator of transition during the G1/S cellular division phase, is deregulated in a wide variety of human cancers. Our group recently reported that overexpression and …
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WebApr 10, 2024 · Stromal cell-derived factor-1 (SDF-1) is a chemokine with a relatively low molecular weight . Two SDF-1 isoforms have been identified: SDF-1α and SDF-1β. ... Results of the CCK-8 assay. (D) and (E) Western blot analysis of cyclin D1 protein expression and statistical analysis of the gray values; ... WebProliferating cell nuclear antigen (PCNA), also known as cyclin, is 29 kDa nuclear protein that plays a crucial role in DNA synthesis (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA also acts as the auxilliary …
WebWestern blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967 (lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2). Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA … WebWestern blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using HSP90 Antibody. Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP90 Antibody. Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using HSP90 Antibody.
WebApr 10, 2024 · Then, the proteins expression of Cyclin E1, 197 Cyclin E2 and CDK2 related to S-phase was tested by western blotting in these cells 198 and obtained the same results (Figure 5C). Assays of wound healing and transwell 199 demonstrated that IGFBP3 significantly impaired the TGF-β-induced enhancement of 200 migration and invasion … WebTET1 Antibodies. Antibodies that detect TET1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, ChIP, Immunocytochemistry and Flow Cytometry. These antibodies target TET1 in Human, Mouse, Rat and Non-human primate samples. Our TET1 polyclonal, monoclonal and recombinant monoclonal …
Web55004-1-AP targets Cyclin B1 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples. Background Information Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
WebApr 13, 2024 · The expression of TMEM40 in CC tissues and cell lines was studied with western blot and real-time quantitative RT-PCR. ... tumor size and weight were significantly lower in the shRNA ... Hellström AC, Andersson S, Zetterberg A. Parallel cyclin E and cyclin A expression in neoplastic lesions of the uterine cervix. Br J Cancer. … lifelabs napanee appointmentWebCyclin E is a prognostic marker in breast cancer, its altered expression increased with the increasing stage and grade of the tumor. Low molecular weight cyclin E isoforms have … lifelabs my visitWebWhy does the observed western blot band size (i.e., molecular weight) differ from the expected band size for some proteins? Typically, SDS-PAGE allows electrophoretic separation of proteins in a sample by molecular weight (MW), with larger proteins migrating more slowly than smaller proteins. lifelabs napanee ontario phone numberWebNov 25, 2024 · cyclin E Antibody (HE12) is an IgG1 κ mouse monoclonal cyclin E antibody suitable for the detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by WB, IP, IF and IHC (P). cyclin E Antibody (HE12) is available as both conjugated and non-conjugated anti-cyclin E antibody forms. mct coursewareWebJun 14, 2024 · cyclin D1 Antibody (A-12) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC (P), FCM and ELISA; may cross-react with cyclin D2; non cross-reactive with cyclin D3 mct course downloadWebThe most frequently used determination method for cyclin E1 is IHC, although western blot allows for assessment of total as well as isoform-specific expression. Cyclin E mRNA … lifelabs napanee ontarioWebQuantitative real-time polymerase chain reaction (PCR) and Western blot analyses were conducted to estimate PER1 mRNA and protein expression. The expression of PER1, P53, CyclinD1, CyclinE, CyclinA2, CyclinB1, cyclin-dependent kinase (CDK) 1, CDK2, CDK4, CDK6, P16, P21, WEE1, and CDC25 mRNA was detected by quantitative real-time PCR. mct cooking oil