2024 By4741 strain

By4741 strain

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August undefined, 2024

WebBy4741 Strain, supplied by Zymo Research, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebBY4741 [ATCC 4040002] Type strain No Genome sequenced strain Yes Isolation source Derived from S288C, ATCC 204508 Product format Frozen Storage conditions -80°C or …

Characterization of plasmid burden and copy number in …

WebJan 21, 2024 · The ability of cocoa powder and CPEX to promote an antioxidant response in the S. cerevisiae BY4741 strain has been previously reported [16,17]. To evaluate whether CPEX also exerted protective antioxidant activity in the deletion mutant strains, we calculated the protecting effect value by dividing the growth ratio curve of the culture ... WebAug 1, 2007 · Surprisingly, it was found that three of the strains that were used, BY4741 (Brachmann et al., 1998), W303 and ΣL5527LH (see Table 1), were sensitive to this … gap to be bridged by the study sample

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WebBY4741 is part of a set of deletion strains derived from S288C in which commonly used selectable marker genes were deleted by design in order to minimize or eliminate … GenBank GCF_000146045.2 Assembly Size 12157105 Scaffolds 17 Longest … WebJan 12, 2007 · Selection of cDNAs Conferring Copper Resistance—Strain BY4741 ace1Δ strain was transformed with a yeast cDNA library (43). Transformants (∼1 × 10 6 colonies) growing on SC-Ura media were collected by resuspending in sterilized distilled water, diluted, and plated on galactose-containing SC-Ura media supplemented with copper … WebApr 13, 2024 · Yeast cells (BY4741 strain background) WT and xrn1-H41A 22 were grown at 30 °C in (1% w/v yeast extract, 2% w/v peptone, 2% w/v glucose/glycerol). All yeast strains used in the present study are ... blackmagic optical fiber

role of FLO11 in Saccharomyces cerevisiae biofilm development in …

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WebWe transformed the arrays into wildtype BY4741 yeast alongside a no-CRISPR control (WT), with the remaining auxotrophic markers introduced on a single-copy plasmid to … WebFeb 23, 2024 · Spot plate assay of the wild-type BY4741 strain (WT) and isogenic mutant sod1Δ (sod1Δ) treated with extracts and ferments (F10 and F20) of R. nigrum, A. melanocarpa, and V. myrtillus and additionally treated with 1 mM hydrogen peroxide for 1 h (WT+ 1 mM H 2 O 2 and sod1Δ + 1 mM H 2 O 2). Strains were serially diluted (as … black magic organicWeb1 day ago · The host strains BY4741 and PLY240 were routinely cultured in YPD (2% glucose; 2% peptone; 1% yeast extract), which was supplemented by 100mM KCl for PLY240. Strains harboring plasmids with the URA3 marker were selected and grown in SD-ura (2% glucose; 0.69% yeast nitrogen base without amino acids; 0.077% CSM Drop-out … black magic othello

"WebOct 6, 2014 · BY4741 (Y00000) yeast wild type strains. Reference: Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, Hieter P and Boeke JD Designer deletion strains derived … " - By4741 strain

By4741 strain

CRISPR-addressable yeast strains with applications in human G …

WebDec 30, 2009 · Abstract. Background: Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and … WebJan 12, 2024 · The BY4741 Δdtd strains containing EcDTD (WT), EcDTD-Mut (A102F), empty vector, and ScDTD constructs were grown in 20 ml of −Leu synthetic-defined broth medium with varying Cu 2+ concentrations overnight at 30°C. The pelleted cells were subjected to the NaOH-TCA precipitation method for protein precipitation. These cultures …

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WebApr 10, 2024 · The LSU rDNA, beginning at the A2 site in ITS1 and terminating after domain VI of 25S, was cloned from the rDNA locus of the Saccharomyces cerevisiae strain BY4741 (MATa his3Δ leu2Δ0 met15Δ0 ... WebNov 1, 2012 · Transformation of BY4741 (MATa) with this vector resulted in a strain with functional control of UAS INO-dependent genes by Opi1p, but strong constitutive …

WebAdditionally, we introduce and validate an enhanced version of the BY4741 yeast strain that contains all four CRISPR-addressable landing pads. We believe this collection of GPCR … WebSpecifically, copy number varied between 2 and 34 copies per cell in the haploid strain and between 4 and 58 copies per cell in the diploid strain. In the BY4741 haploid strain, the …

WebJul 24, 2024 · The two strains, gsh1Δ and BY4741, were cultured in YPD broth medium (10% yeast extract, 10% peptone, 10% glucose and distilled water) overnight to exponential phase (10 −7 cells/mL) at 30 °C. The number of cells/mL (10 −7 cells/mL) of the initial suspension was estimated by cell counting on a Neubauer chamber (Poletto and … WebSpecifically, copy number varied between 2 and 34 copies per cell in the haploid strain and between 4 and 58 copies per cell in the diploid strain. In the BY4741 haploid strain, the low-copy, CEN6/ARSH4 plasmids ranged from 2 to 5 copies per cell and the high copy 2μ plasmids with auxotrophic markers had a wide range of 14 to 34 copies per ...

WebMay 1, 2007 · Abstract. A role of the FLO11 in Saccharomyces cerevisiae biofilm development in a flow cell system was examined. We carried out an ectopic FLO11 expression in the wild type (wt) BY4741 strain that has low levels of endogenous FLO11 transcript. In contrast to the nonadhesive wt, the FLO11 overexpression strain (BY4741 …

WebAug 27, 2010 · Strains. Escherichia coli DH5α was used for the propagation of plasmids; it was grown in Luria broth supplemented with 100 μg of ampicillin per ml when necessary. The S. cerevisiae strain BY4741 (MATa, ura3∆0, leu2∆0,met15∆0, his3∆1) used in this study was obtained from the EUROSCARF collection (Heidelberg, Germany).. Yeast … black magic otoWebAdditionally, we present a BY4741 yeast strain created for broad applications in the yeast and synthetic biology communities that contains only the four CRISPR-addressable landing pads. The general utility of these yeast strains provides an inexpensive, scalable, and easy means of installing and expressing genes directly from the yeast genome ... blackmagic original 2012WebGenomic DNA isolated from Saccharomyces cerevisiae BY4741 [ATCC 4040002]. This fungal strain is also available as ATCC ® Catalog No.: 201388 ™ . Product category … black magic ostWebBY4742 is part of a set of deletion strains derived from S288C in which commonly used selectable marker genes were deleted by design in order to minimize or eliminate … gap to be filledWebYeast Strain By4741, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … blackmagic originalWebFeb 27, 2024 · As in S288c, this strain as well as haploid derivatives BY4741, and BY4742 have allelic variants of MIP1, SAL1 and CAT5 and these polymorphisms, described in … gap to copy and pasteWebFeb 25, 2024 · All strains were from the BY4741 ( his3Δ, ura3Δ, leu2Δ, met15Δ) yeast knockout collection except for art10, which was in the B4742 ( his3Δ, ura3Δ, leu2Δ, lys2Δ) background. Strains were grown on yeast minimal (YM) media supplemented with the necessary nutrients to cover existing auxotrophies in yeast. black magic origin